Study of genetic divergence in Capsicum annuum L. genotypes, using clustering methods
DOI:
https://doi.org/10.5016/1984-5529.2025.v53.1477Keywords:
Singh's Criterion, diversity, Capsicum breeding, upgma, wardAbstract
Capsicum annuum L. is the most cultivated and economically important species in the genus, so studies on genetic divergence are essential. Therefore, the objectives were to evaluate the most promising genotypes for genetic divergence using hierarchical clustering methods and determine which characters are most important in studying C. annuum L. genotypes. The experiment was conducted in a greenhouse at the Departamento de Agronomia, Universidade Federal de Viçosa, using a completely randomized design with four replications. Nine genotypes of C. annuum L. were evaluated based on five quantitative traits. The data were subjected to cluster analysis using the UPGMA and Ward methods, based on the Mahalanobis generalized distance, and the Singh criterion was used to assess the relative importance of each trait for genetic divergence. Therefore, the results indicated that both methods yielded two significant groups, validated by the cophenetic correlation coefficient, and showed good grouping consistency. According to the Singh criterion, the total fruit weight per plant and mature fruit length were the main traits responsible for genetic divergence analysis. Furthermore, the genotypes Pimentão quadrado and Pimenta Doce Italiana exhibited the most satisfactory results for the stated objectives. It can be concluded that these genotypes contribute to obtaining significant gains in a breeding program and guide crosses that possess high genetic variability.
References
Alpiovezza AR, Morais Júnior SLA, Gonçalves ID, Netto AAL, Marcucci MC (2015) Pimentas do gênero Capsicum: ações farmacológicas e propriedades terapêuticas. Revista de Fitoterapia 15 (2): 121-130.
Bianchi PA, Dutra IP, Moulin MM, Santos JO, Santos Júnior AC (2016) Morphological characterization and analysis of genetic variability among pepper accessions. Ciência Rural 46 (7): 1151-1157. https://doi:10.1590/0103-8478cr20150825
Cruz EA, Camacho EA, Jimenez SLR, Ávila ALV, Alfonso LF, Hernández AYP (2018) Efecto de la aplicácion combinada de fertilizante químico y húmus de lombriz em Capsicum annuum. Revista Centro Agrícola 45 (1): 52-61.
Cruz CD (2013) GENES - a software package for analysis in experimental statistics and quantitative genetics. Acta Scientiarum. 35 (3): 271-276.
Cruz CD, Carneiro PCS, Regazzi AJ (2014) Modelos biométricos aplicados ao melhoramento genético. Editora UFV. 668 p.
Cruz CD, Ferreira FM, Pessoni LA (2020) Biometria aplicada ao estudo de diversidade genética. UFV. 626 p.
Ferreira DF (2018) Estatística multivariada. Editora UFLA, 2008. 624 p.
Mahalanobis PC (1936) On the generalized distance in statistics. Proceedings of The National Institute of Sciences of India 12: 49-55.
Mojena R (1977) Hierarquical grouping methods and stopping rules: na evaluation. The Computer Journal 20: 359-363.
Moll RH, Lonnquist JH, Fortuno JV, Johnson EC (1965) The relationship of heterosis and genetic divergence in maize. Genetics 52 (1):139-144.
Pinto KMS, Barbosa LA, Ferreira DS, Nascimento LC, Rêgo ER, Bruno RLA (2014) Sanidade e fisiologia de sementes de pimenta Cambuci Capsicum baccatum L. var pendulum tratadas como extrato de Lippia microphylla. Bioscience Journal 30 (3): 743-749.
Pinto Júnior BB, Nunes LF, Kuplich MMD, Martins MG (2015) Aplicabilidade da pimenta (capsaicina) na estética. Revista Interdisciplinar de Estudos em Saúde 4 (2): 109-119. https://doi:10.33362/ries.v4i2.719
Puiatti GA, Cecon PR, Nascimento M, Nascimento ACC, Finger FL, Puiatti M, Silva FF, Silva AR (2014) Comparação dos métodos de agrupamento de Tocher e UPGMA no estudo de divergência genética em acessos de alho. Revista da Estatística UFOP 3 (3): 275-279.
R Development Core Team (2021) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Áustria.
Regazzi AJ, Cruz CD (2020) Estatística multivariada aplicada. UFV. 408 p.
Ribeiro CSC, Carvalho SIC, Reifschneider FJB, Bianchetti LB, Lopes CA, Lima MF, Reis A, Duval AMQ, Pinheiro JB, Nagata AKI, Ragassi CF (2020) Cultivares de pimentas das espécies Capsicum spp. desenvolvidas pela Embrapa Hortaliças. Embrapa. 30 p.
Rosário VNM, Chaves RPF, Pires IV, Filho AFS, Toro MJU (2021) Capsicum annuum e Capsicum chinense: características físicas, físico-químicas, bioativas e atividade antioxidante. Brazilian Journal of Development 7 (5): 50414-50432. https://doi:10.34117/bjdv7n5-441
Silva Neto JJ, Rêgo ER, Nascimento MF, Silva Filho VAL, Almeida Neto JX, Rêgo MM (2014) Variabilidade em população base de pimenteiras ornamentais (Capsicum annuum L.). Revista Ceres 61 (1): 84-89. https://doi:10.1590/S0034-737X2014000100011
Singh D (1981) The relative importance of characters affecting genetic divergence. The Indian Journal Of Genetic and Plant Breeding 41 (2): 237-245.
Sneath PHA, Sokal RR (1973) Numerical Taxonomy: the principles and practice of numerical classification. W H Freeman & Co. 588 p.
Vaz Patto MC, Satovic Z, Pêgo S, Fevereiro P (2004) Assessing the genetic diversity of Portuguese maize germoplasm using microsatellite markers. Euphytica 137(1): 63-72.
Zamljen T, Vesna Z, Slatnar A (2020) Influence of Irrigation on Yield and Primary and Secondary Metabolites in Two Chilies Species, Capsicum Annuum L. and Capsicum Chinense Jacq. Agricultural Water Management 234 (3): 1-7. https://doi:10.1016/j.agwat.2020.106104
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Copyright (c) 2025 Thaynara Aparecida de SOUZA NETO, Paulo Roberto CECON, Ana Carolina Ribeiro de OLIVEIRA, Sebastião MARTINS FILHO, Wellington SOUTO RIBEIRO
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