Biopriming in black oat seeds

Abstract

This study performs the microbiolization of black oat seeds with Trichoderma spp. and Bacillus subtilis by physio­logical conditioning, suspension of biological structures, and film coating, improving the control of pathogens and the effect on seed germination and vigor. Microbiolization with suspension of biological structures was carried out with commercial products based on Trichoderma spp. and Bacillus subtilis. Water restriction was performed in PDA + Mannitol (-0.8 MPa) medium, for Trichoderma spp. and B. subtilis. One hundred disinfected seeds of black oat were distributed in each plate. Once root protrusion occurred in one seed, the others were removed and dried, under laboratory conditions, for 48h. Film coating was performed with the addition of polymer to the treatment syrup containing Trichoderma spp. or B. subtilis. The seeds were dried for 48 h in a laboratory environment. A treatment was used to coat the conditioned seeds with the organisms, individually or in combination. Trichoderma spp. and B. subtilis control the pathogens associated with black oat seeds. B. subtilis increases the germination, seedling performance and growth, and dry matter accumulation of black oat. Trichoderma spp. promotes seedling shoot growth and dry matter accumulation in black oat plants. However, Trichoderma spp. and B. subtilis, applied by physiological conditioning and film coating, compromise the germination and emergence of black oat seed­lings.